TABLE 1.
Recovery of mixed-species suspensions of C. parvum and C. hominis oocysts subjected to the two-color FISH assaya
Ratio (%) of C. parvum to C. hominis oocysts | Mean % recovery following two-color FISH
|
|
---|---|---|
C. parvum | C. hominis | |
10:90 | 6.1 ± 2.2 | 80.7 ± 5.8 |
25:75 | 18.7 ± 5.1 | 68 ± 4.3 |
50:50 | 40.5 ± 5.0 | 41.1 ± 4.8 |
75:25 | 69.2 ± 4.1 | 18.8 ± 3.2 |
90:10 | 82.3 ± 4.9 | 6.8 ± 2.0 |
C. parvum- and C. hominis-specific probes were used for the detection of viable oocysts. Oocysts were quantified using epifluorescence microscopy.