FIG. 3.

LSPA of 14 of the clade strains. Fourteen E. coli O157:H7 strains from eight clades were subjected to LSPA. The positions of the six gene markers (folD-sfmA, Z5395, yhcG, rtcB, rbsB, and iclR-arp) are indicated on the left. A molecular size marker (50 bp) was loaded in the middle, and sizes are indicated on the right. Strain names and clade designations are indicated below the gel. FRIK920 and EDL933, which were previously reported as LSPA genotype 222222 and LSPA genotype 111111, respectively (45), were used as standards for fragment migration rates. Data from this figure were used to construct Table 6.