TABLE 1.
Growth, carbohydrate consumption, and metabolite production of Anaerostipes caccae DSM 14662T, Roseburia faecis DSM 16840T, Roseburia hominis DSM 16839T, Roseburia intestinalis DSM 14610T, and Roseburia inulinivorans DSM 16841T in mMCB supplemented with 50 mM FE fructose, oligofructose, or inulin or 100 mM lactate
| Strain | Mean ± SD consumption (mM) of substrate (after 48 h)
|
Mean ± SD production (mM) of metabolite (after 48 h)
|
Carbon recovery (%) | Electron recovery (%) | Substrate depletion time (h) | Max. cell populationf (time [h] max. was reached) | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Energy source | Acetate | Butyrate | Carbon dioxide | Hydrogen gas | Lactate | |||||
| A. caccae DSM 14662T | 50.2 ± 0.1a | 28.7 ± 1.0 | 58.6 ± 0.4 | 104.0 ± 0.1 | 67.4 ± 0.1 | 0 | 94.3 | 91.0 | 24-48 | 2.7 (24) |
| A. caccae DSM 14662T | 86.1 ± 1.4b | 39.9 ± 1.8 | 63.2 ± 0.4 | 85.3 ± 0.1 | 42.8 ± 0.1 | 0 | 100 | 99.8 | >48 | 1.8 (9) |
| R. faecis DSM 16840T | 38.8 ± 0.2a | 0 | 30.1 ± 0.5 | 84.6 ± 0.1 | 90.1 ± 0.1 | 0 | 88.1 | 84.0 | >48 | 1.1 (6) |
| R. faecis DSM 16840T | 12.8 ± 0.2c | 6.5 ± 1.0 | 15.0 ± 0.4 | 23.4 ± 0.1 | 13.6 ± 0.1 | 0 | 92.6 | >48 | 1.1 (3) | |
| R. hominis DSM 16839T | 50.9 ± 0.2a | 20.8 ± 0.9 | 55.7 ± 0.1 | 95.2 ± 0.1 | 65.1 ± 0.1 | 7.0 ± 0.2 | 99.4d | 96.5d | 15-24 | 4.4 (15) |
| R. intestinalis DSM 14610T | 51.8 ± 1.3a | 36.8 ± 1.1 | 74.5 ± 0.9 | 103.5 ± 0.1 | 57.7 ± 0.1 | 0 | 104.4 | 104.4 | 15-24 | 3.1 (15) |
| R. intestinalis DSM 14610T | 56.0 ± 0.2c | 33.0 ± 0.4 | 67.5 ± 0.5 | 101.7 ± 0.1 | 62.9 ± 0.1 | 0 | 91.7 | >48 | 2.2 (48) | |
| R. inulinivorans DSM 16841T | 46.4 ± 0.4a | 31.1 ± 0.7 | 35.7 ± 0.2 | 38.9 ± 0.1 | 0 | 50.4 ± 0.3 | 97.7 | 96.8 | >48 | 1.5 (15) |
| R. inulinivorans DSM 16841T | 52.3 ± 0.2c | 22.0 ± 0.6 | 28.6 ± 0.7 | 30.1 ± 0.1 | 0 | 66.7 ± 0.6 | 96.3 | 15-24 | 2.8 (15) | |
| R. inulinivorans DSM 16841T | 45.8 ± 0.6e | 31.0 ± 1.1 | 35.5 ± 0.8 | 40.9 ± 0.1 | 0 | 51.0 ± 0.2 | 99.7 | >48 | 2.0 (24) | |
a
The energy source was fructose.
b
The energy source was lactate.
c
The energy source was oligofructose (FE).
d
Traces of formate (6.0 ± 0.3 mM) were included in the calculation.
e
The energy source was inulin (FE).
f
Determined by monitoring the optical density at 600 nm.