TABLE 2.
Comparisons between calculated and measured butyrate, CO2, and H2 productiona
| Strain (fermentation substrate) | Production level (mM)
|
|||||
|---|---|---|---|---|---|---|
| Butyrate
|
CO2
|
H2
|
||||
| Calculated | Measured | Calculated | Measured | Calculated | Measured | |
| A. caccae DSM 14662T (fructose) | 64.6 | 58.6 | 100.5 | 104.0 | 71.8 | 67.4 |
| A. caccae DSM 14662T (lactate) | 63.0 | 63.2 | 86.1 | 85.3 | 46.2 | 42.8 |
| R. faecis DSM 16840T (fructose) | 38.8 | 30.1 | 77.7 | 84.6 | 77.7 | 90.1 |
| R. faecis DSM 16840T (oligofructose) | 16.0 | 15.0 | 25.6 | 23.4 | 19.2 | 13.6 |
| R. hominis DSM 16839T (fructose) | 57.8 | 55.7 | 94.8 | 95.2 | 74.0 | 65.1 |
| R. intestinalis DSM 14610T (fructose) | 70.2 | 74.5 | 103.7 | 103.5 | 66.9 | 57.7 |
| R. intestinalis DSM 14610T (oligofructose) | 73.0 | 67.5 | 113.1 | 101.7 | 80.1 | 62.9 |
| R. inulinivorans DSM 16841T (fructose) | 36.8 | 35.7 | 42.4 | 38.9 | 11.3b | 0.0 |
| R. inulinivorans DSM 16841T (oligofructose) | 30.0 | 28.6 | 38.0 | 30.1 | 16.0b | 0.0 |
| R. inulinivorans DSM 16841T (inulin) | 35.8 | 35.5 | 40.6 | 40.9 | 9.6b | 0.0 |
a
Based on fructose/oligofructose/inulin, lactate, and acetate consumption and lactate production by clostridial cluster XIVa butyrate producers in mMCB supplemented with 50 mM FE fructose, oligofructose, or inulin or 100 mM lactate (Fig. 1; Table 1). Calculations are based on the metabolic pathway proposed in Fig. 2.
b
Theoretical (Fig. 2) and measured H2 production levels were 0; deviant calculated values are to be attributed to measuring errors for input data (fructose/oligofructose/inulin and acetate consumption or lactate production).