FIG. 2.

Phylogenetic tree of 1,502 Bacteroidales 16S rRNA gene sequences derived from different animal hosts and from water, lagoon, and manure samples, based on a neighbor-joining algorithm. Numbers in parentheses indicate the number of sequences associated in each clade for a given host. Sequences for cultured Bacteroidales members were added to the analyses as reference points. MPOH, manure pit sample from Ohio; PigOH, pig fecal sample from Ohio; PWOH, water sample impacted with pig feces from Ohio; SSIL, sample from a stream south of a site in Illinois; LGIL, lagoon sample from Illinois; TN, OR, NE, JP, and TX, samples taken from Tennessee, Oregon, Nebraska, Japan, and Texas, respectively; CC, chicken cecum; 163, clones generated using PF163 and Bac708 primers; A5, E4, C7, and E8, samples taken from groundwater wells from Illinois sites A, C, and E.