TABLE 1.
Description of primers tested in this study
| Primer name | Primer sequence | Target | Reference |
|---|---|---|---|
| P23-2f | 5′-TCTGCGACACCGGTAGCCATTGA-3′ | mcrA gene of methanogens | 35 |
| P23-2r | 5′-ATACACTGGCGACATTCTTGAGGATTAC-3′ | ||
| Bac32F | 5′-AACGCTAGCTACAGGCTT-3′ | 16S rRNA gene of Bacteroidales | 2 |
| Bac708R | 5′-CAATCGGAGTTCTTCGTG-3′ | ||
| PigBac1f | 5′-CGGGTTGTAAACTGCTTTTATGAAG-3′ | 16S rRNA gene of Prevotella-related group | 29 |
| PigBac1r | 5′-CGCTCCCTTTAAACCCAATAAA-3′ | ||
| PF163 | 5′-GCGGATTAATACCGTATGA-3′ | 16S rRNA gene of Prevotella-related group | 12 |
| Bac708R | 5′-CAATCGGAGTTCTTCGTG-3′ |