TABLE 4.
Proportion of positive PCR results using general Bacteroidales and swine-associated markers on water DNA extracts
| Water sampling site (n) | No. of positive PCR results/total no. of PCR results at DNA template concn
|
|||||
|---|---|---|---|---|---|---|
| General Bacteroidales (Bac32)
|
Swine-associated Prevotella (PF163)
|
Swine-associated methanogens (P23-2)
|
Swine-associated Prevotella (PigBac1)
|
|||
| 0.2 ng | 1 ng | 1 ng | 10 ng | 50 ng | 10 ng | |
| Illinoisa | ||||||
| Site A | ||||||
| Wells (16) | 6/16 | 7/16 | 1/16 | 3/16 | 1/16 | 2/16 |
| Lagoon (1) | 1/1 | 0/1 | 0/1 | 1/1 | 0/1 | 1/1 |
| North stream (1) | 1/1 | 1/1 | 0/1 | 0/1 | 0/1 | 0/1 |
| North tile (1) | 1/1 | 0/1 | 0/1 | 0/1 | 0/1 | 0/1 |
| Site C | ||||||
| Wells (6) | 3/6 | 1/6 | 2/6 | 2/6 | 2/6 | 1/6 |
| Lagoon (1) | 1/1 | 1/1 | 1/1 | 1/1 | 0/1 | 1/1 |
| Seep from field (1) | 1/1 | 1/1 | 0/1 | 1/1 | 0/1 | 0/1 |
| South stream (1) | 1/1 | 1/1 | 1/1 | 1/1 | 0/1 | 0/1 |
| Site E | ||||||
| Wells (11) | 5/11 | 6/11 | 4/11 | 3/11 | 0/11 | 0/11 |
| Manure pit (n = 1) | 1/1 | 1/1 | 0/1 | 1/1 | 0/1 | 1/1 |
| Texas | ||||||
| Lake Granbury (10) | 10/10 | NDb | ND | 6/10 | 2/10 | 10/10 |
| Buck Creek (10) | 10/10 | ND | ND | 6/10 | 0/10 | 0/10 |
a
With use of the general bacterial 16S rRNA gene-targeted PCR assay (8F/787R), 37/40 environmental DNA extracts from Illinois yielded positive PCR results.
b
ND, not determined.