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. 2009 Jul 31;191(19):5941–5952. doi: 10.1128/JB.00778-09

FIG. 1.

FIG. 1.

Deletion, complementation and overexpression of Rv0348. (A) Organization of the Rv0348 operon and our strategy for gene disruption. See Materials and Methods for details. (B) Southern blot analysis of SalI-digested genomic DNA of the H37Rv wild type and the ΔmosR mutant. (C) PCR analysis of cDNA synthesized from RNA samples purified from H37Rv (lanes 1, 3, and 5) or the ΔmosR mutant (lanes 2, 4, and 6). (D) Western blot analysis of different M. tuberculosis strains using polyclonal antibodies raised in rabbits against the MBP-MosR protein. Pellets from M. tuberculosis H37Rv (lane 1), the ΔmosR mutant (lane 2), the ΔmosR::mosR complemented strain (lane 3), and the H37Rv::mosR overexpression strain (lane 4) were subjected to immunoblotting. The blot for soluble fractions was negative (data not shown).