FIG. 4.
Transcriptional activity of the mosR operon. (A) EMSA of MosR with different promoter regions of significantly regulated genes as detected by DNA microarrays. EMSA was performed as described previously (44). Gene names are listed above the lanes. (B) Western blot analysis of the recombinant strain of M. smegmatis mc2155 expressing MosR protein. (C) β-Galactosidase activities of constructs for Rv3130c (tgs1), Rv0347, Rv0700, and Rv0167 promoters fused to the coding sequence of lacZ in the presence/absence of the mosR operon using M. smegmatis cells as host cells. The asterisks denote significant changes in a Student t test (P < 0.001). The error bars indicate standard deviations.