TABLE 1.
Vectors and constructs used in this study
| Plasmid | Characteristics | Reference |
|---|---|---|
| pYUB854 | Cosmid for disruption construct | 2 |
| pMV361 | Integrative mycobacterial shuttle vector; Kanr | 39 |
| M. smegmatis::pML21 | Recombinant M. smegmatis harboring the whole MosR operon under the control of its own promoter, in addition to hsp60; Kanr | This work |
| pML23 | pMV361 harboring the MosR gene; Kanr | This work |
| pCV77 | Replicative shuttle vector with promoterless LacZ transcriptional fusion; Kanr | MedImmune |
| pML24 | pCV77 with hygromycin cassette in opposite direction to LacZ.; Kanr Hygr | This work |
| pML25 | pML24 harboring the promoter region of the Rv0347 operon; Kanr Hygr | This work |
| pML26 | pML24 harboring the promoter region of Rv0167 (mce1 operon); Kanr Hygr | This work |
| pML27 | pML24 harboring the promoter region of Rv0700 (rpsJ); Kanr Hygr | This work |
| pML28 | pML24 harboring the promoter region of Rv3130c (tgs1); Kanr Hygr | This work |
| pML29 | pML24 harboring the promoter region of hsp60; Kanr Hygr | This work |