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. 2009 Jul 31;191(19):6167–6177. doi: 10.1128/JB.01605-08

TABLE 5.

Partial purification of butyryl-CoA dehydrogenase

Purification step Protein amt (mg) Sp act (U/mg) Yield (%) Purification factor
Cell extract 72 NDa NAb NA
Soluble fraction 45.4 12.19 100 1
DEAE-Sepharose CL-6B (pH 8.0) 5.14 40.72 37.8 3.34
Membrane fraction 28.8 0.11 100 1
Solubilized membrane protein 14.16 0.53 236 4.81
DEAE-Sepharose CL-6B (pH 8.0) 0.765 14.19 342 129
a

ND, not determined.

b

NA, not applicable.