TABLE 5.
Purification step | Protein amt (mg) | Sp act (U/mg) | Yield (%) | Purification factor |
---|---|---|---|---|
Cell extract | 72 | NDa | NAb | NA |
Soluble fraction | 45.4 | 12.19 | 100 | 1 |
DEAE-Sepharose CL-6B (pH 8.0) | 5.14 | 40.72 | 37.8 | 3.34 |
Membrane fraction | 28.8 | 0.11 | 100 | 1 |
Solubilized membrane protein | 14.16 | 0.53 | 236 | 4.81 |
DEAE-Sepharose CL-6B (pH 8.0) | 0.765 | 14.19 | 342 | 129 |
ND, not determined.
NA, not applicable.