Location of all ACA triplets in tRNA and 5S rRNA sequences of E. coli K-12. (A) Nucleotide sequences (Seq) of any tRNAs containing ACAs are shown (for a total of 10 unique ACA-containing tRNAs), with the predicted single-strand and double-strand (Str) regions indicated by dots and arrowheads, respectively. ACA triplets are underlined, and the methylation site of is marked with an arrow. (B) The nucleotide sequence of the 5S rRNA gene, rrfF, is similarly shown.