FIG. 6.

Induction of MazF cleaves tmRNA at single-strand ACA sites. Primer extensions were run on total RNA from strains containing pBR322 alone (−), wild-type tmRNA (wt), or triple mutant tmRNA (+aca) over a time course of 30 min. (Left) Cleavage at ACA81 (boxed) in the triple mutant but not in the wild type or vector alone. (Right) Cleavage at ACA188 in the triple mutant only but no cleavage at ACA278 with any of the vectors. Full-length (FL) primer extension products are marked with triangles. Single asterisks indicate primer extension products ending at single-strand ACA sites. The double asterisk indicates incomplete primer extension, ending at regions of stable secondary structure.