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MazF cleavage occurs at ACA sites. Sequencing reactions for tmRNA were run next to primer extension reactions using total RNA harvested 0 and 30 min after MazF induction. (Left) Cleavage at A82C. (Right) Cleavage at C188A. Part of the proteolysis tag-encoding region of tmRNA is shown in brackets. Single asterisks indicate minor cleavage bands, while double asterisks indicate major cleavage bands.
