FIG. 2.
Effect of nickel and cobalt on growth of ARY023 strains of E. coli. Overnight cultures of ARY023 (open triangle), MC4100 (filled triangle), ARY023/pUCmrdH (filled circle), and ARY023/pUCrcnAPsy (open circle) in M9 MM supplemented with 0.4% glucose were diluted into 1:100 fresh identical medium with the indicated concentrations of Ni2+ (A) and Co2+ (B). Cell growth was monitored as the OD600 after 16 h of incubation at 37°C. Experiments were performed in triplicate, and the values given are averages.