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Expression of mreA in E. coli ARY023. Overnight cultures of ARY023/pUC18 (open circle) and ARY023/pUCmreA (filled circle) in M9 MM supplemented with 0.4% glucose were diluted into 1:100 fresh identical medium with the indicated concentrations of metals (Cd2+ and Ni2+). Cell growth was monitored as the OD600 after 16 h of incubation at 37°C.
