TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Genotype or characteristics | Source or reference |
|---|---|---|
| Strains | ||
| P. putida | ||
| KT2440 | Wild type | 35 |
| HMR020 | KT2440 mrdH::ltrB | This study |
| E. coli | ||
| DH5α | F− φ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17 (rK− mK+) phoA supE44 λ−thi-1 gyrA96 relA1 | Lab collection |
| JM109 | endA1 recA1 gyrA96 thi-1 hsdR17(rK− mK+) Δel4(ΔmcrA) supE44 relA1 Δ(proAB lac) F′ [proAB+traD36 lacIqlacZΔM15] | 58 |
| ARY023 | F supE thi Δ(lac-proAB) hsd5/F′ proAB+lacIqlacZΔM15 yohM::uidA-Kanr | 46 |
| Plasmids | ||
| pTZ57R/T | Ampr, cloning vector | Fermentas |
| pUC18 | ColE1 replicon; Ampr; 2.7 kb | 58 |
| pUCmrdH | pUC18 containing mrdH gene of P. putida KT2440 | This study |
| pUCrcnAPsy | pUC18 containing the rcnAPsy gene of P. syringae DC3000 | This study |
| pUCmreA | pUC18 containing mreA gene of P. putida KT2440 | This study |
| pJB653 | Ampr; 7.0 kb, derivative of pJB652 in which the PstI fragment containing the Pm promoter was cloned in the opposite orientation | 3 |
| pJBmrdH | pJB653 containing the mrdH gene of P. putida KT2440 | This study |
aAmpr, ampicillin resistance; Kanr, kanamycin resistance.