TABLE 2.
Sequences of the primers used in PCR amplifications
| Primer pair | Sequence (5′-3′)a |
|---|---|
| A1 | CCCGGG ATGTCGAGCTTCGCTGAACTG |
| AAGCTT AGGCCGATCCAGCCATGGA | |
| A2 | CCCGGG ATGCCCGACTTTGCCAGTCTG |
| AAGCTT ACAGACCATTCCAGCCGTG | |
| A3 | CCCGGG ATGAGCGATCACGAACACG |
| AAGCTT TAGAGGTACTTAGTGATTTGCTTGAA | |
| A4 | CCCGGG ATGTCGAGCTTCGCTGAACTG |
| GGATCC AGGCCGATCCAGCCATGGA | |
| A5 | GTAAGCGTTCGGTCGAGAAG |
| CCACCGATGTACGAGGAAAC | |
| A6 | ATGCTGAAGCACAACACCAG |
| TTTAGCAATCGCAACGTCAC | |
| A7 | TGGTCTTGGATAAGCACACG |
| GCCATCAGCACCAAAGTCAT | |
| A8 | GTGTTTGCCTTCACCCATCT |
| CCAGTGGGTTTCGATCAAGT | |
| A9 | GATTCTGTTGCAGCGACTCA |
| GACCAATTCGATGCCTGTCT | |
| A10 | TAACTGGAAGCCAGCCAAAC |
| GAAGTGCTTGCTGTCATGGA |
a
Restriction sites are indicated by underlining.