TABLE 3.
Determination of MTCs for P. putida KT2440 and derivative strainsa
| Metal | MTC (mM) for P. putida strain:
|
||
|---|---|---|---|
| KT2440 | HMR020/pJB653 | HMR020/pJBmrdH | |
| Cd2+ | 0.2 | 0.01 | 0.2 |
| Ni2+ | 4 | 1 | 4 |
| Zn2+ | 4 | 1 | 4 |
| Co2+ | 0.6 | 0.4 | 0.6 |
| Cu2+ | 2 | 2 | 2 |
| Pb2+ | >1.0 | >1.0 | >1.0 |
| Mn2+ | >1.5 | >1.5 | >1.5 |
a
P. putida KT2440 (wild type), HMR020/pJB653. and HMR020/pJBmrdH strains were grown till early log phase (OD600 = 0.3) in LB medium at 30°C, diluted 1:100 in fresh medium, and spotted onto LB agar plates containing the indicated concentrations of Cd2+, Zn2+, Ni2+, and Co2+.