TABLE 4.
Expression analysis of mrdH and mreA in P. putida KT2440
| Metala | Concn (mM) | Mean fold change ± SDb
|
|||
|---|---|---|---|---|---|
|
mrdH
|
mreA
|
||||
| LB | M9 MM | LB | M9 MM | ||
| Cd2+ | 0.02 | 2.2 ± 0.15 | 1.18 ± 0.10 | 3.7 ± 0.10 | 1.40 ± 0.15 |
| 0.2 | 2.5 ± 0.10 | 2.58 ± 0.15 | 4.0 ± 0.15 | 2.27 ± 0.10 | |
| Ni2+ | 0.1 | 1.0 ± 0.20 | 3.89 ± 0.15 | 1.27 ± 0.2 | 2.4 ± 0.20 |
| 1.0 | 2.2 ± 0.21 | 5.5 ± 0.20 | 3.0 ± 0.15 | 4.28 ± 0.30 | |
| Zn2+ | 0.05 | 1.03 ± 0.05 | 1.19 ± 0.15 | 1.03 ± 0.2 | 1.10 ± 0.15 |
| 0.5 | 1.36 ± 0.10 | 7.78 ± 0.25 | 1.36 ± 0.2 | 4.14 ± 0.25 | |
| Co2+ | 0.1 | 1.56 ± 0.15 | 2.29 ± 0.10 | 1.27 ± 0.05 | 2.14 ± 0.15 |
| 1.0 | 2.0 ± 0.05 | 1.00 ± 0.20 | 2.0 ± 0.20 | 1.40 ± 0.20 | |
a
P. putida KT2440 was grown till early log phase in LB/M9 MM and then induced with the indicated metals for 40 min.
b
The values indicate the fold change in expression of the mrdH and mreA genes in the presence of various metals.