TABLE 5.

Expression analysis of tnpA (Tn4652), tnpC (Tn4652), and IS1246 in P. putida KT2440

Metala	Concn (mM)	Mean fold change ± SDb
		tnpA (Tn4652)		tnpC (Tn4652)		IS1246
		LB	M9 MM	LB	M9 MM	LB	M9 MM
Zn2+	0.05	2.8 ± 0.30	8 ± 0.35	1 ± 0.2	1.10 ± 0.15	1.1 ± 0.25	8 ± 0.15
	0.5	5.3 ± 0.40	16 ± 0.40	1.4 ± 0.2	1.14 ± 0.20	2.42 ± 0.2	8 ± 0.2
Ni2+	0.1	2.46 ± 0.18	1.4 ± 0.15	1.27 ± 0.10	2.3 ± 0.10	1.31 ± 0.2	2.5 ± 0.1
	1.0	4.92 ± 0.10	2.5 ± 0.10	1.62 ± 0.15	2.5 ± 0.25	1.50 ± 0.15	4 ± 0.2
Cd2+	0.02	1.1 ± 0.10	1.62 ± 0.10	1.0 ± 0.15	1.40 ± 0.15	1.5 ± 0.1	2.2 ± 0.15
	0.2	2.51 ± 0.23	2.5 ± 0.25	1.15 ± 0.12	1.27 ± 0.13	1.62 ± 0.1	2.54 ± 0.10
Co2+	0.1	1.51 ± 0.15	1.40 ± 0.30	1.27 ± 0.15	1.14 ± 0.10	1.40 ± 0.15	1.6 ± 0.15
	1.0	1.60 ± 0.25	1.75 ± 0.45	1.51 ± 0.2	1.40 ± 0.2	1.60 ± 0.2	1.8 ± 0.2

^aP. putida KT2440 was grown till early log phase in LB/M MM and then induced with the indicated metals for 40 min.

^bThe values indicate the fold change in expression of the tnpA, tnpC, and IS1246 genes in the presence of various metals.