TABLE 4.
Strain | Genotype | CFUa
|
% Sporulation (±SD) | |
---|---|---|---|---|
Preheat | Postheat | |||
PY79 | Wild type | 5.5 × 108 | 5.0 × 108 | 91 ± 5.7 |
JDB2327 | ΔytgP::mls | 3.5 × 108 | 3.3 × 108 | 90 ± 2.6 |
JDB2330 | ΔykvU::mls | 4.7 × 108 | 4.5 × 108 | 89 ± 4.7 |
JDB2386 | yabM::cm | 3.7 × 108 | 3.5 × 108 | 91 ± 2.7 |
JDB1097 | spoVBΔ::tet | 1.6 × 108 | 0 | 0 |
JDB2329 | spoVB-gfp spc | 4.8 × 108 | 4.4 × 108 | 90 ± 2.4 |
JDB2437 | spoVBΔ::tet amyE::Psweet-spoVB-gfp spc | 3.3 × 108 | 3.0 × 108 | 91 ± 4.9 |
JDB2506 | amyE::Psweet-mviN spc | 3.7 × 108 | 3.5 × 108 | 92 ± 4.4 |
JDB2504 | spoVBΔ::tet amyE::Psweet-mviN spc | 3.9 × 108 | 2.4 × 108 | 60 ± 5.8 |
Strains were sporulated in DSM for 24h at 37°C. Cultures were diluted, and CFU were counted before and after exposure to 80°C for 20 min.