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. 2009 Jul 24;191(19):5910–5920. doi: 10.1128/JB.00292-09

FIG. 5.

FIG. 5.

UV survival of GW8017 strains harboring plasmids expressing UmuD variants (A) G129S and (B) D84N. (A) Noncleavable UmuD(G129S) suppresses UV sensitivity that is characteristic of noncleavable UmuD variants. UmuD(G129S) also suppresses the extreme UV sensitivity of UmuD(S60A). •, pGY9739-umuDC-D129 (pD129); ▪, pGY9738-umuD′C (pUmuD′C); −, pGB2 (pVector); □, pGY9739- umuDC-DS60A, G129S (pD129-S60A); ⧫, pGY9739-umuDC (pUmuDC); ▴, pGY9739-umuDC-DS60A (pS60A). (B) UmuD(D84N) is cleavable, and strains harboring plasmids expressing this variant are resistant to UV light. A strain expressing UmuD(S60A D84N) is sensitive to UV light, which is characteristic of a strain expressing UmuD(S60A). ▪, pGY9738-umuD′C (pUmuD′C); −, pGB2 (pVector); ⧫, pGY9739-umuDC (pUmuDC); •, pGY9739-umuDC-D84 (pD84); □, pGY9739-umuDC-D84, S60A (pD84-S60A); ▴, pGY9739-umuDC-DS60A (pS60A).