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Peptide array blot showing selected UmuC residues and their corresponding mutations, probed with wild-type β protein. Only the UmuC residues predicted to be surface exposed are shown. The blots on the left contained the wild-type UmuC sequences shown, and the blots on the right contained the sequences with the underlined residue mutated, as indicated in Table 2. The “X” indicates a spot that is not meaningful, because the antibody control reacted at this position (data not shown).
