TABLE 2.
Nucleotide sequence analysis of umuD′C alleles containing missense mutations
| Plasmid | Nucleotide substitutiona | Deduced amino acid substitutionb |
|---|---|---|
| pGYHA-1 | G732A (TGC→TAC) | UmuC (Cys105Tyr) |
| G841A (CAG→CAA) | Silent | |
| pGYHA-2 | G384A (GGT→AGT) | UmuD′ (Gly129Ser) |
| G841A (CAG→CAA) | Silent | |
| pGYHA-3 | C1583T (CTC→TTC) | UmuC (Leu389Phe) |
| pGYHA-5 | C1146T (ACG→ATG) | UmuC (Thr243Met) |
| pGYHA-6 | G463C (GAG→GAC) | UmuC (Glu15Asp) |
| C665T (CGG→TGG) | UmuC (Arg83Trp) | |
| pGYHA-7 | C494T (CCG→TCG) | UmuC (Pro26Ser) |
| C510T (TCG→TTG) | UmuC (Ser31Leu) | |
| pGYHA-9 | G497A (GTG→ATG) | UmuC (Val27Met) |
| G503A (GTG→ATG) | UmuC (Val29Met) | |
| pGYHA-10 | G249A (GAT→AAT) | UmuD′ (Asp84Asn) |
| pGYHA-11 | G778A (GGC→AGC) | UmuC (Gly120Ser) |
| G1107A (CGC→CAC) | UmuC (Arg230His) |
a
For nucleotide numbering the full-length first codon of UmuD (ATG) was considered position 1.
b
Amino acid numbering begins with position 1 for each protein (UmuD or UmuC).