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. 2009 Jul 31;191(19):6012–6019. doi: 10.1128/JB.00604-09

FIG. 4.

FIG. 4.

Moenomycin sensitivity of mutant strains. (A) Cultures in exponential growth phase were inoculated into multiwell plates containing medium with various concentrations of moenomycin. One hundred to 200 cells were inoculated into 250 μl of medium. OD values were determined after 16 h at 37°C and are expressed as percentages of that obtained in the absence of moenomycin. Results for PS832 (wild type, ⋄), DPVB488 (ΔyabM, •), DPVB450 (ΔykvU, ▴), DPVB489 (ΔytgP, ▪), and DPVB521 (ΔyabM ytgP::MLS, □) are shown. Values are averages determined for two independent cultures, with error bars indicating standard deviations. Where error bars are not visible, the standard deviations are <2%. (B) Cultures in exponential growth phase were diluted into fresh medium without (open symbols and ×) or with (filled symbols and ✻) moenomycin, and OD was monitored during shaking at 37°C. Results for PS832 (wild type, ⋄ and ♦), DPVB488 (ΔyabM, ○ and •), DPVB521 (ΔyabM ytgP::MLS, □ and ▪), DPVB532 (ΔyabM ΔytgP ykvU::MLS, × and ✻), and DPVB615 (ΔyabM amyE::xylP-yabM, ▴) are shown. Complementation of ΔyabM was observed during growth of DPVB615 in the presence of 0.01% xylose.