TABLE 1.
Bacillus subtilis strains
| Strain | Genotype | Constructiona | Source |
|---|---|---|---|
| PS832 | Wild type | Setlow lab | |
| DPVB327 | ykvU::MLS | pDPV221→PS832 | This work |
| DPVB335 | yabM::MLS | pDPV220→PS832 | This work |
| DPVB336 | ytgP::MLS | pDPV222→PS832 | This work |
| DPVB450 | ΔykvU | pDPV283→PS832b | This work |
| DPVB488 | ΔyabM | pDPV337→PS832b | This work |
| DPVB489 | ΔytgP | pDPV338→PS832b | This work |
| DPVB520 | ΔyabM ykvU::MLS | DPVB327→DPVB488 | This work |
| DPVB521 | ΔyabM ytgP::MLS | DPVB336→DPVB488 | This work |
| DPVB522 | ΔytgP ykvU::MLS | DPVB327→DPVB489 | This work |
| DPVB523 | ΔytgP yabM::MLS | DPVB335→DPVB489 | This work |
| DPVB532 | ΔyabM ΔytgP ykvU::MLS | DPVB489+DPVB327→DPVB488 | This work |
| DPVB614 | ΔytgP amyE::xylP-ytgP | pDPV411→DPVB489 | This work |
| DPVB615 | ΔyabM amyE::xylP-yabM | pDPV412→DPVB488 | This work |
a
The designation preceding the arrow indicates the source of donor DNA in a natural transformation of the recipient strain following the arrow.
b
The indicated transformation was unique and the first in a series of steps in introduction of the in-frame deletion, as described in Materials and Methods.