TABLE 1.
Strain | Genotype | Constructiona | Source |
---|---|---|---|
PS832 | Wild type | Setlow lab | |
DPVB327 | ykvU::MLS | pDPV221→PS832 | This work |
DPVB335 | yabM::MLS | pDPV220→PS832 | This work |
DPVB336 | ytgP::MLS | pDPV222→PS832 | This work |
DPVB450 | ΔykvU | pDPV283→PS832b | This work |
DPVB488 | ΔyabM | pDPV337→PS832b | This work |
DPVB489 | ΔytgP | pDPV338→PS832b | This work |
DPVB520 | ΔyabM ykvU::MLS | DPVB327→DPVB488 | This work |
DPVB521 | ΔyabM ytgP::MLS | DPVB336→DPVB488 | This work |
DPVB522 | ΔytgP ykvU::MLS | DPVB327→DPVB489 | This work |
DPVB523 | ΔytgP yabM::MLS | DPVB335→DPVB489 | This work |
DPVB532 | ΔyabM ΔytgP ykvU::MLS | DPVB489+DPVB327→DPVB488 | This work |
DPVB614 | ΔytgP amyE::xylP-ytgP | pDPV411→DPVB489 | This work |
DPVB615 | ΔyabM amyE::xylP-yabM | pDPV412→DPVB488 | This work |
The designation preceding the arrow indicates the source of donor DNA in a natural transformation of the recipient strain following the arrow.
The indicated transformation was unique and the first in a series of steps in introduction of the in-frame deletion, as described in Materials and Methods.