TABLE 2.
Bacillus subtilis plasmids and oligonucleotides
| Plasmid | Oligonucleotidea | Sequenceb | Vector |
|---|---|---|---|
| pDPV220 | DLP179 | CGGAATTCCACCATATTAAAAATATCAGC | pMUTIN4 |
| DLP180 | CGGGATCCTCCCGCAGTATAAAGCGAGGC | pMUTIN4 | |
| pDPV221 | DLP175 | CGGAATTCAATCCTAAGTTTCATGAAAGC | pMUTIN4 |
| DLP176 | CGGGATCCGAAATCAGCACAGCCATGTCC | pMUTIN4 | |
| pDPV222 | DLP177 | CGGAATTCAAAGGGGATTATGAGACAAGC | pMUTIN4 |
| DLP178 | CGGGATCCACTCAATAGAAAGATGATGCG | pMUTIN4 | |
| pDPV283 | DLP311 | GCTCTAGAAGTCTGAAAAAGAGTTGC | pBKJ236 |
| DLP312 | CTTCAATTCGCGGGGATCCCACAAATCGATTCATGATG | pBKJ236 | |
| DLP313 | GAATCGATTTGTGGGATCCCCGCGAATTGAAGGATG | pBKJ236 | |
| DLP314 | GAACTGCAGGTGATGATATGATACTCC | pBKJ236 | |
| pDPV337 | DLP307 | GCTCTAGAAGTGCTGATTTGTGATCG | pBKJ236 |
| DLP308 | CCATTTTGTTCGGATCCTATTGAATCGTCCATCC | pBKJ236 | |
| DLP309 | GGACGATTCAATAGGATCCGAACAAAATGGCGGGTAA | pBKJ236 | |
| DLP310 | GAACTGCAGGAAGCCGTCATTTGGTCG | pBKJ236 | |
| pDPV338 | DLP315 | GCTCTAGACTTCCTTCAATTGAATGC | pBKJ236 |
| DLP316 | CTGCATGTCTGCCGGATCCCAGTTTGCTTGACATGTTTC | pBKJ236 | |
| DLP317 | GTCAAGCAAACTGGGATCCGGCAGACATGCAGGCTG | pBKJ236 | |
| DLP318 | GAACTGCAGATCTGTGATTTTAAATGG | pBKJ236 | |
| pDPV411 | DLP468 | GCGCTTAATTAAATGGTGATGAAGAAACATGTC | pSWEET-bgaB |
| DLP469 | GCGCGGATCCTCATCAGCCTGCATGTCTGCC | pSWEET-bgaB | |
| pDPV412 | DLP466 | GCGCTTAATTAAGGGAAGGAGCTTTTGGATGG | pSWEET-bgaB |
| DLP467 | GCGCGGATCCCGACGACTGTAATTTTACCC | pSWEET-bgaB |
a
The two or four oligonucleotides listed for each plasmid were used to perform PCR as described in Materials and Methods to produce fragments that were cloned into the indicated vector.
b
Underlined sequences indicate restriction sites used to clone PCR products to the indicated vector. Bold sequences indicate the BamHI sites inserted within in-frame deletion sites.