TABLE 2.
Plasmid | Oligonucleotidea | Sequenceb | Vector |
---|---|---|---|
pDPV220 | DLP179 | CGGAATTCCACCATATTAAAAATATCAGC | pMUTIN4 |
DLP180 | CGGGATCCTCCCGCAGTATAAAGCGAGGC | pMUTIN4 | |
pDPV221 | DLP175 | CGGAATTCAATCCTAAGTTTCATGAAAGC | pMUTIN4 |
DLP176 | CGGGATCCGAAATCAGCACAGCCATGTCC | pMUTIN4 | |
pDPV222 | DLP177 | CGGAATTCAAAGGGGATTATGAGACAAGC | pMUTIN4 |
DLP178 | CGGGATCCACTCAATAGAAAGATGATGCG | pMUTIN4 | |
pDPV283 | DLP311 | GCTCTAGAAGTCTGAAAAAGAGTTGC | pBKJ236 |
DLP312 | CTTCAATTCGCGGGGATCCCACAAATCGATTCATGATG | pBKJ236 | |
DLP313 | GAATCGATTTGTGGGATCCCCGCGAATTGAAGGATG | pBKJ236 | |
DLP314 | GAACTGCAGGTGATGATATGATACTCC | pBKJ236 | |
pDPV337 | DLP307 | GCTCTAGAAGTGCTGATTTGTGATCG | pBKJ236 |
DLP308 | CCATTTTGTTCGGATCCTATTGAATCGTCCATCC | pBKJ236 | |
DLP309 | GGACGATTCAATAGGATCCGAACAAAATGGCGGGTAA | pBKJ236 | |
DLP310 | GAACTGCAGGAAGCCGTCATTTGGTCG | pBKJ236 | |
pDPV338 | DLP315 | GCTCTAGACTTCCTTCAATTGAATGC | pBKJ236 |
DLP316 | CTGCATGTCTGCCGGATCCCAGTTTGCTTGACATGTTTC | pBKJ236 | |
DLP317 | GTCAAGCAAACTGGGATCCGGCAGACATGCAGGCTG | pBKJ236 | |
DLP318 | GAACTGCAGATCTGTGATTTTAAATGG | pBKJ236 | |
pDPV411 | DLP468 | GCGCTTAATTAAATGGTGATGAAGAAACATGTC | pSWEET-bgaB |
DLP469 | GCGCGGATCCTCATCAGCCTGCATGTCTGCC | pSWEET-bgaB | |
pDPV412 | DLP466 | GCGCTTAATTAAGGGAAGGAGCTTTTGGATGG | pSWEET-bgaB |
DLP467 | GCGCGGATCCCGACGACTGTAATTTTACCC | pSWEET-bgaB |
The two or four oligonucleotides listed for each plasmid were used to perform PCR as described in Materials and Methods to produce fragments that were cloned into the indicated vector.
Underlined sequences indicate restriction sites used to clone PCR products to the indicated vector. Bold sequences indicate the BamHI sites inserted within in-frame deletion sites.