TABLE 4.

Mutant phenotypic properties

Strain	Genotype	Doubling time (min)a	No. of heat-resistant sporesb		Avg cell length (μm)c	% Cells in chains ofc:
			T8	T24		2	>2	>8
PS832	Wild type	19 ± 1	2.5 × 108	3.1 × 108	4.1 ± 1.1	58	0	0
					3.9 ± 1.0	44	2	0
DPVB488	ΔyabM	19 ± 1	3.5 × 108	5.0 × 108	4.6 ± 1.3	54	0	0
					3.9 ± 0.9	53	0	0
DPVB450	ΔykvU	20 ± 2	1.2 × 108	5.3 × 108	4.6 ± 1.4	47	2	0
					4.2 ± 2.3	39	0	0
DPVB520	ΔyabM ΔykvU	19 ± 1	8.3 × 107	4.2 × 108	4.5 ± 1.2	69	0	0
					3.8 ± 0.9	47	0	0
DPVB489	ΔytgP	20 ± 2	2.7 × 107*	9.7 × 107	6.4 ± 1.9**	49	42	19
					5.7 ± 1.7**	74	17	0
DPVB614d	ΔytgP amyE::xylP-ytgP	20 ± 2	2.4 × 108	ND	4.5 ± 1.4	53	2	0
DPVB521	ΔyabM ytgP::MLS	21 ± 2	4.3 × 106*	3.6 × 107	6.5 ± 1.5**	57	39	20
					6.3 ± 3.1**	40	52	0
DPVB522	ΔytgP ykvU::MLS	20 ± 1	1.4 × 107*	9.4 × 107	7.3 ± 2.4**	37	45	19
					5.7 ± 1.4**	82	9	0
DPVB532	ΔyabM ΔytgP ykvU::MLS	22 ± 1	2.5 × 106*	2.8 × 107	6.9 ± 1.8**	45	45	26
					5.6 ± 1.7**	61	22	0

^aValues are averages determined for three independent cultures ± standard deviations.

^bValues are averages determined for three independent cultures. T₈, 8 h after entry into stationary phase; T₂₄, 24 h after entry into stationary phase. *, value is significantly different (P ≤ 0.05 using unpaired Student's t test) than that observed for the wild-type strain.

^cFor each strain, the first value is for a mid-log culture (OD of 0.5) and the second value is for a late log culture (OD of 1.5). Average cell lengths are for 200 cells, and standard deviations are given. Cells in chains of >8 are also included in chains of >2. **, value is significantly different (P < 0.0001 using unpaired Student's t test) than that observed for the wild-type strain.

^dThis strain was assayed for ytgP complementation only during mid-log growth with 0.01% xylose.