FIG. 7.

Primer extension analysis of dppA transcript. A primer extension reaction mixture with total RNA was prepared from cultures of P. putida KT2442 (wild type [wt]) and MPO201 (ΔntrC) grown on minimal medium under nitrogen-sufficient (NS, ammonium plus serine) or nitrogen-limiting (S, serine) conditions. GATC lanes indicate the sequencing ladder (noncoding strand). A 13-nucleotide sequence around the transcriptional start (coding strand) is shown. Arrows indicate the mapped 5′ ends of the transcripts.