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Variant promoter constructs used in the study. Map of the tgs1-Rv3131 intergenic region. P and S sites are represented by gray shaded and white rectangular boxes, respectively. Putative −10 and −35 promoter elements are indicated by small black boxes. Various promoter variants used in this study are shown below the map. Nucleotides mutated in P and S boxes are aligned with WT nucleotides. N, WT sequence.
