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Effect of P site and S site mutations on in vitro DNA binding and in vivo transcriptional activation of tgs1 and Rv3131. (A) DNase I footprinting with phosphorylated DevR (DevR∼P) and WT, S mut, P mut, and PS mut DNAs. (B) GFP reporter assay. The GFP fluorescence of M. tuberculosis standing cultures (48 h) is expressed as RFU/OD at 595 nm ± standard deviation.
