TABLE 1.
Plasmids used in this study
| Plasmid | Relevant featuresa | Reference |
|---|---|---|
| pFPV27 | E. coli-mycobacterial shuttle plasmid with promoterless gfp; Kmr | 28 |
| pTGS | pFPV27 containing tgs1 promoter (−143 to +45); Hygr | This study |
| p3131 | pFPV27 containing Rv3131 promoter (−150 to +38); Hygr | This study |
| pTGS-P mut | pTGS containing mutated P DevR binding site; Hygr | This study |
| pTGS-S mut | pTGS containing mutated S DevR binding site; Hygr | This study |
| pTGS-PS mut | pTGS containing mutated P and S DevR binding site; Hygr | This study |
| p3131-P mut | p3131 containing mutated P DevR binding sites; Hygr | This study |
| p3131-S mut | p3131 containing mutated S DevR binding site; Hygr | This study |
| p3131-PS mut | p3131 containing mutated P and S DevR binding site; Hygr | This study |
| pTGS P-inv | pTGS containing inverted P DevR binding site; Hygr | This study |
| p3131 P-inv | p3131 containing inverted P DevR binding site; Hygr | This study |
| pTGS-ΔS | pTGS with S DevR binding site deletion; Hygr | This study |
| p3131-ΔS | p3131 with S DevR binding site deletion; Hygr | This study |
a
Promoter coordinates are with reference to the TSP.