TABLE 1.
E. coli strains and plasmids used in this study
| Strain or plasmid | Relevant genotype and/or phenotype | Reference or source |
|---|---|---|
| Strains | ||
| CS85 | ruvC53 eda-51::Tn10 | 68 |
| DM49 | lexA3(Ind−) malB::Tn9 | 45 |
| FC29 | Δ(lac-proAB)XIIIthi ara [F′ proAB+ Δ(lacI-lacZ)] | 8 |
| FC526 | ΔrecG263::Kan | 18 |
| JW2711 | ΔrpoS::FRTKanFRT | 2 |
| N2731 | recG258::Tn10mini-Kan | 47 |
| RW120 | ΔumuDC595::cat | 79 |
| SMR580 | FC40 recB21 argA::Tn10 | 24 |
| SMR593 | FC40 recB21 | 24 |
| SMR4562 | Δ(lac-pro)XIIIthi ara Rifr [F′ α45 lacIqlacI33ΩlacZ] (genotype identical to that of FC40, independent construction) | 52 |
| SMR5201 | Δattλ::ampRC | 61 |
| SMR5222 | SMR4562 Δattλ::ampRC | 61 |
| SMR5225 | SMR5222 Δ(srlR-recA)306::Tn10 | 61 |
| SMR5228 | SMR5222 recB21 argA::Tn10 | SMR5222 × P1(SMR580) |
| SMR5522 | FC29 ΔruvC64::Kan | 7 |
| SMR5578 | SMR5222 ΔrecG263::Kan | 61 |
| SMR5652 | SMR5222 ΔrecG263::Kan Δ(srlR-recA)306::Tn10 | 61 |
| SMR6064 | SMR4562 dinB10 (λxis1 cIts857) [F′ dinB10] | 53 |
| SMR6371 | SMR5222 recB21 | SMR5228 × P1(SMR593) |
| SMR6373 | SMR4562 dinB10 Δattλ::ampRC [F′ dinB10] | SMR6064 × P1(SMR5201) |
| SMR6485 | SMR5222 ruvC53 eda-51::Tn10 | SMR5222 × P1(CS85) |
| SMR6487 | SMR5222 recB21 recG258::Tn10mini-Kan | SMR6371 × P1(N2731) |
| SMR6927 | SMR5222 ΔrecG263::Kan ruvC53 eda-51::Tn10 | SMR5578 × P1(CS85) |
| SMR7055 | SMR5222 ΔrecG263::Kan lexA3(Ind−) malB::Tn9 | SMR5578 × P1(DM49) |
| SMR10316 | SMR5222 ΔumuDC595::cat | SMR5222 × P1(RW120) |
| SMR10317 | SMR5222 ΔrpoS::FRTKanFRT | SMR5222 × P1(JW2711) |
| Plasmids | ||
| pJP2 | pTGV-Light ampRC+ | 61 |
| pJP19 | pACYC184 ampD+ | 61 |
| pKD46 | ori101 repA101(Ts) pBAD-gam-bet-exo AmpraraC+ on plasmid | 13 |