TABLE 2.
Oligonucleotides used in this study
| Primer | Sequence (5′-3′)a |
|---|---|
| NdeI-F-pga | GGGCCCCATATGTCGTATTTTACAAATGACA |
| BmtI-R-pga | GGGCCCGCTAGCACTTGACATAATAAACAGATAATCAG |
| XhoI-R-pga | GGGCCCCTCGAGCAAAGACCAAATTGCAAACTACAAA |
| BmtI-F-pag | GGGCCCGCTAGCCACTCATGAAAAATGATGCAAATATC |
| Pga-1F | GAGCAGGACAAACTCAACAAGCTGT |
| Pga-1R | AAAAAGCAGCCACATTAAACAAAACCAACC |
| BbvCI-Ori-F | GGGCCCCCTCAGCGATCGTAGAAATATCTATGATTA |
| BbvCI-Ori-R | GGGCCCCCTCAGCGGATTTTAACATTTTGCGTTGTTCCAA |
| Pga-comp-F | AGTGTCAGATGGAGTTGCGG |
| Pga-comp-R | CTTTTGACGCCCAATACCGCACGGG |
| 2159-F | GCAAACTACAAACTTGGCCGATTGT |
| pgaD-R | TATGACTATGTGTGGGTAGATCATGTTTCAG |
| pgaD-F | CTGAAACATGATCTACCCACACATAGTCATA |
| pgaC-R | GCCGCAGTGGTATGGCGTGATATTAGGT |
| pgaC-F | TCTAGGATTACCGGTTACAGCACCAATTCT |
| pgaB-R | GCCCTTTGCAGGTCTCGC |
| pgaB-F | TTGCATTTCTCGCACTTGCTTCCAATTGAC |
| pgaA-R | GGCCTTTATAAACAGGCCGATTACTCTGCT |
| pgaA-F | ACCGATAATAAAATACGCCCATCAACTGAC |
| 2163-R | AGTGTCAGATGGAGTTGCGG |
a
The restriction sites introduced into oligonucleotides are underlined.