TABLE 1.
Gene ID | Deduced function | Sequence of CebR boxa | Positionb | Location | Transcription level (ΔcebR/wt)c |
---|---|---|---|---|---|
SGR199 | Secreted cellulose-binding protein | TGGGAGCGCTCCCA, CGGGAGCGCTCCCC | −324.5, −519.5 | TL | 39 |
SGR217 | Secreted pectate lyase | TGGGAGCGCTCCCG | −103.5 | TL | 4.2 |
SGR1971 | Secreted protein of unknown function | TGGGAGCGCTCCCC | −298.5 | TL | 5.5 |
SGR2445 | Secreted cellulase | TGGGAGCGCTCCCA | −23.5 | TL | 15 |
SGR3391 | Acetyltransferase | CGGGAGCGCTCCCG | −25.5 | TL | 1.6 |
SGR4735 | cebE; cellobiose ABC transporter solute-binding protein | TGGGAGCGCTCCCA | +13.5 | TC | 10 |
SGR4738 | bglC; β-glucosidase | AGGGAGCGCTCCCA | −8.5 | TL | 5.8 |
SGR4739 | cebR; LacI/GalR family transcriptional regulator | TGGGAGCGCTCCCA | −21.5d | TC | -e |
SGR6927f | Hypothetical protein | TGGGAGCGCTCCCA, TTGGAGCGCTCCCA | −296.5, −209.5 | TL | 2.6 |
SGR6928f | Secreted cellulase | TGGGAGCGCTCCAA, TGGGAGCGCTCCCA | −199.5, −112.5 | TL | 130 |
Nucleotides that match the consensus CebR box sequence are underlined.
Nucleotide position of the center of the CebR box relative to the transcriptional start site (TC) or the translational start site (TL).
Average value from three quantitative-RT-PCR experiments. wt, wild type.
From the transcriptional start site of cebRp1 (Fig. 5E).
Not detected because the cebR coding region was deleted in the ΔcebR mutant.
SGR6927 and SGR6928 are divergent. Two CebR boxes exist in the intergenic region between SGR6927 and SGR6928.