TABLE 2.

Nucleotide sequences of oligonucleotide primers used for sequencing, PCR-based cloning, mutagenesis procedures, and transcriptional analysis in this study

Primer	Primer sequence (5′-3′)a	Gene specificity
913F	TGGGCATTGTATCAAGGGC	Sequencing and cloning of lgt4
914R	ATTGGGTGGGTAGATTCTGTG	Sequencing and cloning of lgt4
915F	CTCGAGAGCACCCAAAGCAAATCAGAGATG	lgt4 mutagenesis
916R	AGATCTGCCCAATACAGCCCATTATGAAC	lgt4 mutagenesis
508F	AAGAAGTGGGGCTTTTGTCAGAG	Mutagenesis and cloning of lgt2B/C mutant in 26391
509R	GAGAGTATGTCATTCGTGGCGAC	Mutagenesis and cloning of lgt2B/C mutant in 26391
1102R	GTCATCAGCATAAAAACTGC	Sequencing and cloning of lgt2A from 25238
1103F	ATAGCGATTCGTTGGC	Sequencing and cloning of lgt2A from 25238
1104F	AGATCTCGGTGAGTCTTTGGTTGTTGA	Mutagenesis of lgt2A from 25238
1105R	CTCGAGTCAGGTGCTAAGCGGCTACTA	Mutagenesis of lgt2A from 25238
1086F	TTCATTGCCGATACCACC	Sequencing and cloning of lgt1 from 25238; lgt1 transcription
1098R	AAACCGCATAACTGTCTGAC	Sequencing and cloning of lgt1 from 25238
1099R	AGATCTCACTGTTACTGTGCTCTGTGC	Mutagenesis of lgt1 from 25238
1100F	CTCGAGATCTTTCCTGTGTTGTGGCA	Mutagenesis of lgt1 from 25238
1264F	ATTTTGGGGCAGGCAAG	Sequencing and cloning of lgt5
1265R	TGGATTGGTGATACACTCGG	Sequencing and cloning of lgt5
1297R	CTCGAGCTGCCCAAAGAAAGTGAGT	lgt5 mutagenesis
1298F	AGATCTCAGAAAATAACGCATAACTGCC	lgt5 mutagenesis
1087R	TTACTCCGCCAACCATTCC	lgt1 transcription
649F	ATCCTGCTCCAACTGACTTTC	lgt2A transcription
650R	GGTAACAGAACGCTCAACCC	lgt2A transcription
671F	GTGTCATCTTCTGATACTAAATGGG	lgt2B/C transcription
672R	AAACTTTTCATACAGGCACTGC	lgt2B/C transcription

^aBglII restriction sites are in italics, and XhoI restriction sites are underlined.