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Growth phase-dependent changes in B. bronchiseptica TTSS-mediated cytotoxicity as measured by percent LDH release. J774 macrophages treated with medium alone (M) or infected with RB50 or RB50ΔbscN from cultures collected in mid-log phase (15 h), late log phase (24 h), and stationary phase (48 h) for 4 h at an MOI of 10. The error bars represent standard deviations. An asterisk indicates that the P value is <0.001.
