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Growth phase-dependent changes in B. bronchiseptica adherence to L2 epithelial cells. L2 cells were incubated with RB50, RB63 (ΔfimBCD), RBX9 (ΔfhaB), or SP5 (Δprn) from cultures collected in mid-log phase (15 h), late log phase (24 h), and stationary phase (48 h) for 40 min at an MOI of 100. Adherence is expressed as the proportion of adherent bacteria to that in the original inoculum. The error bars represent standard deviations. An asterisk indicates that the P value is <0.05.
