TABLE 2.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| P. gingivalis strains | ||
| ATCC 33277 | Wild type | ATCC |
| W83 | Wild type | Gift from M. J. Duncana |
| TDC60 | Wild type | Gift from K. Ishiharab |
| TDC117 | Wild type | |
| TDC275 | Wild type | |
| SU63 | Wild type | Gift from M. Yonedac |
| GAI-7802 | Wild type | Gift from E. Hoshinod |
| KDP136 | kgp-2::cat rgpA2::[ermFermAM] rgpB2::tetQ | 19 |
| KDP377 | kgp-2::cat rgpA2::[ermFermAM] rgpB2::tetQ Δ[gslA]::cepA | This study |
| KDP378 | kgp-2::cat rgpA2::[ermFermAM] rgpB2::tetQ Δ[pgm6]::cepA | This study |
| KDP379 | kgp-2::cat rgpA2::[ermFermAM] rgpB2::tetQ Δ[λσθβ]pgm6pgm7]::cepA | This study |
| E. coli strains | ||
| XL-1 Blue | General-purpose host strain for recombinant protein production | Stratagene |
| BL21(DE3) | Host strain for production of recombinant proteins | 20 |
| Plasmids | ||
| pCR4Blunt-TOPO | Apr Kmr; plasmid vector for cloning | Invitrogen |
| pBluescript ΙΙ SK(+) | Apr; plasmid vector for cloning | Stratagene |
| pET22b | Apr; plasmid vector for cloning | Novagenf |
| pCS22 | Apr; contains the cepA DNA cassette at the AatII site of pCS14 | Gift from E. C. Reynoldse |
| pKD1001 | Apr Kmr; contains 0.6-kb upstream and 0.9-kb downstream gslA DNA fragments in pCR4BluntII-TOPO | This study |
| pKD1002 | Apr Kmr; contains the cepA DNA cassette at the BamHI site of pKD1001 | This study |
| pKD1003 | Apr; contains 0.8-kb upstream and 0.8-kb downstream pgm6 DNA fragments in pBluescript ΙΙ SK(+) | This study |
| pKD1004 | Apr; contains 0.8-kb upstream and 0.8-kb downstream pgm7 DNA fragments in pBluescript ΙΙ SK(+) | This study |
| pKD1005 | Apr; contains the cepA DNA cassette at the BamHI site of pKD1003 | This study |
| pKD1006 | Apr; contains the cepA DNA cassette at the BamHI site of pKD1004 | This study |
| pKD1007 | Apr; contains the 5′-terminal third of gslA in pET22b | This study |
a
Department of Molecular Genetics, The Forsyth Institute, Boston, MA.
b
Department of Microbiology, Tokyo Dental College, Chiba, Japan.
c
Department of General Dentistry, Fukuoka Dental College, Fukuoka, Japan.
d
Department of Oral Health Science, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan.
e
Cooperative Research Centre for Oral Health Science, School of Dental Science, University of Melbourne, Victoria, Australia.
f
San Diego, CA.