TABLE 2.
Primer pairs used for real-time RT-PCR analysis of genes
| Genea | Primer sequence |
|---|---|
| GAPDH | 5′-TGTAGACCATGTAGTTGAGGTCA-3′ |
| 5′-AGGTCGGTGTGAACGGATTTG-3′ | |
| IFN-γ | 5′-TCAAGTGGCATAGATGTGGAAGAA-3′ |
| 5′-TGGCTCTGCAGGATTTTCATG-3′ | |
| IL-6 | 5′-GAGGATACCACTCCCAACAGACC-3′ |
| 5′-AAGTGCATCATCGTTGTTCATACA-3′ | |
| IL-10 | 5′-GGTTGCCAAGCCTTATCGGA-3′ |
| 5′-ACCTGCTCCACTGCCTTGCT-3′ | |
| IL-12 | 5′-GGAAGCACGGCAGCAGAATA-3′ |
| 5′-AACTTGAGGGAGAAGTAGGAATGG-3′ | |
| iNOS | 5′-TTGGGTCTTGTTCACTCCACGG-3′ |
| 5′-CCTCTTTCAGGTCACTTTGGTAGG-3′ | |
| KC | 5′-TGCACCCAAACCGAAGTCAT-3′ |
| 5′-TTGTCAGAAGCCAGCGTTCAC-3′ | |
| MIP-3α | 5′-CCAGGCAGAAGCAAGCAACT-3′ |
| 5′-TCGGCCATCTGTCTTGTGAA-3′ | |
| MIP-2 | 5′-AGTGAACTGCGCTGTCAATGC-3′ |
| 5′-AGGCAAACTTTTTGACCGCC-3′ | |
| TNF-α | 5′-CATCTTCTCAAAATTCGAGTGACAA-3′ |
| 5′-TGGGAGTAGACAAGGTACAACCC-3′ |
a
GAPDH, glyceraldehyde-3-phosphate dehydrogenase; IFN-γ, gamma interferon; TNF-α, tumor necrosis factor alpha.