TABLE 1.
Plasmids
| Plasmid | Relevant characteristicsa | Reference or origin |
|---|---|---|
| pUC18 | Cloning vector, Apr | 42 |
| pT7Blue | Commercial cloning vector, Apr | Novagen |
| pJIR750 | E. coli-C. perfringens shuttle vector, carries pIP404 replication region, Cmr | 4 |
| pJIR751 | E. coli-C. perfringens shuttle vector, carries pIP404 replication region, Emr | 4 |
| pJIR2783 | Mobilizable base suicide vector for clostridia, Cmr Emr | 11 |
| pJIR2812 | pJIR2783 (HindIII/XbaI)ΩJRP2015/JRP2016 (HindIII/XbaI, 2.4 kb) PCR product, Cmr Emr | This study |
| pJIR2822 | pJIR2212 (PstI/BamHI)ΩJRP2049/JRP2050 (PstI/BamHI, 2.4 kb) nanI suicide vector, Cmr Emr | This study |
| pJIR2958 | pT7BlueΩJRP2152/JRP2153 (3.5 kb) nanJ+, Apr | This study |
| pJIR3057 | pJIR751 (PstI/XbaI)ΩpJIR3046 (PstI/XbaI 2.6 kb) nanI+ complementation plasmid, Emr | This study |
| pJIR3109 | pMOD-3 R6Kγori/MCS (Epicentre)Ωerm(B), Apr Emr | This study |
| pJIR3181 | pJIR2958 EZ-Tn5 mutant at nanJ nucleotide 2153, nanJ suicide vector, Apr Emr | This study |
| pJIR3270 | pJIR750 (BamHI), pJIR2593 (BamHI, 4 kb) tet(M), Tcr Cmr | This study |
| pJIR3308 | pJIR2958 (SmaI)ΩpJIR3270 (SmaI, 8.2 kb) tet(M), catP, C. perfringens rep and ori, nanJ+ complementation vector, Tcr Cmr Apr | This study |
| pJIR3309 | pJIR3248 (SmaI)ΩpJIR3270 (SmaI, 8.2 kb) tet(M), catP, C. perfringens rep and ori, Tcr Cmr Apr | This study |
a
Apr, Cmr, Emr, Tcr, Rifr, and Nalr, resistance to ampicillin, chloramphenicol (thiamphenicol), erythromycin, tetracycline, rifampin, and nalidixic acid, respectively.