TABLE 3.
Plasma creatine kinase activity after infection with C. perfringens mutants
| Strain | Properties | Creatine kinase activity (IU/liter)a |
|---|---|---|
| JIR325 | Wild-type strain 13 Rifr Nalr | 4,970 ± 840 |
| JIR325 | Wild type (heat-killed cells) | 201 ± 45 |
| JIR4915 | JIR325 ΔnanI::catP, nanI mutant (Rifr Nalr Cmr) | 5,080 ± 750 |
| JIR12017 | JIR4915 nanJ::erm(B), nanIJ double mutant (Rifr Nalr Cmr Emr) | 5,020 ± 680 |
| JIR12018 | JIR325 nanJ::erm(B), nanJ mutant (Rifr Nalr Emr) | 5,370 ± 810 |
a
Myotoxicity was evaluated after intramuscular injection (n = 8), and the creatine kinase activity in plasma was determined 5 h later. The values are the averages and standard deviations for at least three independent experiments. One international unit is the amount of enzyme that transforms 1 μmol of substrate per min under standard conditions.