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Transcriptional analysis of the MYPE9110 gene. M. penetrans cells were grown in SP-4 medium, and RNA was isolated at 3, 6, 12, 18, 24, and 30 h of growth. RT-PCR (n = 30 cycles) was used to confirm MYPE9110 and tufA mRNA syntheses in M. penetrans GTU-54, and the corresponding cDNA was amplified and resolved by 1% agarose gel electrophoresis.
