Growth effects of separately or simultaneously depleting MPR1/2 and RBP16 in BF cells. (A) BF T. brucei cell lines harboring RNAi constructs against RBP16; both MRP1 and MRP2 (MRP1/2); or MRP1, MPR2, and RBP16 (MRP1/2-RBP16) were grown in the absence (−TET) or presence (+TET) of tetracycline, and growth was monitored over 12 days. (B) MRP1, MRP2, and RBP16 protein levels in the cell lines analyzed in panel A were determined by Western blotting. HSP70 protein levels were monitored as a loading control.