FIG. 1.

AIP2 expression enhances T-cell activation. (A) Mouse primary T cells were infected with retrovirus carrying AIP2, Itch, or GFP only as a control. Infected cells were cultivated with anti-CD3 or anti-CD3 plus anti-CD28 for 2 days. Partial supernatants were collected from each well for the analysis of IL-2 production by enzyme-linked immunosorbent assay. (B) Cells were then chased with 0.5 μCi [³H]thymidine (³H-TdR) for an additional 12 h, and the [³H]thymidine incorporation was analyzed. Error bars represent data from three independent experiments (means ± standard deviations). (C) Infected cells were stained with phycoerythrin-conjugated annexin V. GFP-positive cells were gated for the analysis of annexin V, and representative data are shown. (D) The average percentages of annexin V-positive cells from three independent experiments are shown. The Student t test was used for the statistic analysis.