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. 2009 Aug 3;29(19):5357–5365. doi: 10.1128/MCB.00302-09

FIG. 2.

FIG. 2.

AC76E is a functional adenylate cyclase and mediates dFoxO-regulated cAMP levels in S2 cells. (A) Overexpression of AC76E in S2 cells (bar/lane 2) increases the cAMP concentration compared to results for the empty vector control (bar/lane 1). Forskolin was added to both samples. (B) Overexpression of dFoxO-A3 alone was sufficient to increase the cAMP concentration in S2 cells compared to results for the empty vector control (lanes/bars 1 and 2). Forskolin was added to both samples. (C) The increase in the cAMP concentration upon dFoxO-A3 overexpression was attenuated by RNAi against AC76E (upper histogram, bars 2 and 3). No forskolin was added. The efficiency of the knockdown was measured by qPCR for AC76E mRNA (lower histogram, bars 1 and 2). The error bars in each histogram represent standard deviations from at least two independent measurements.