FIG. 3.

Expression of ac76e during fly development. (A) The temporal expression level of ac76e was measured by qPCR analysis in different stages of fly development: E, embryo; L1, first instar; L2, second instar; L3, third instar; P1, pupa 1; P2, pupa 2; female, adult female; male, adult male. (B to G) dFoxO regulates ac76e expression in third-instar gastric cecum and CA: mRNA in situ staining of stage 16 embryo (B), third-instar gastric cecum, and ring gland (C to G). ac76e in situ with antisense (B to D) or sense (E) probe or dfoxo in situ with anti-sense (F) or sense (G) probe is shown. Fly strains used were EP-147/EP-147 (+/+) and the homozygous mutant dFoxO (dfoxo²⁵/dfoxo²⁵). (H to I) cAMP levels measured from third-instar ring gland (H) or gastric cecum (I) from dfoxo²⁵/dfoxo²⁵ and dfoxo²⁵/+ animals. (J) Expression analysis of ac76e and 4ebp from fed and starved dfoxo²⁵/dfoxo²⁵ and dfoxo²⁵/+ animals. The P values are derived from a one-tailed Student t test. The error bars represent standard deviations from at least three independent measurements. *, P < 0.05 (Student's t test).