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. 2009 Aug 3;29(19):5357–5365. doi: 10.1128/MCB.00302-09

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FIG. 4. — Overexpression of AC76E in the CA delays pupa formation and reduces adult body weight. (A) DI-11-Gal4 drives expression specifically in the CA as shown by GFP activity in a DI-11-Gal4/UAS-GFP third-instar ring gland. (B) Western blot from whole adult fly extracts showing the expression of the UAS-AC76E-HA P-element insertions driven by tubulin-Gal4. (C) Pupa formation in flies of genotypes w¹¹¹⁸; +/DI-11-Gal4, w¹¹¹⁸; UAS-AC76E^3rd/DI-11-Gal4, and w¹¹¹⁸; +/UAS-AC76E^2nd; +/DI-11-Gal4. (D) Left: a 165-h-AEL larva of genotype w¹¹¹⁸; +/UAS-AC76E^2nd; +/DI-11-Gal4; right: a 120-h-AEL larva of genotype w¹¹¹⁸; DI-11-Gal4/+. (E) Adult flies of genotypes w¹¹¹⁸; DI-11-Gal4/+ (right) and w¹¹¹⁸; +/UAS-AC76E^2nd; +/DI-11-Gal4 (left). The AC76E-overexpressing flies on the left originate from larvae still wandering at 165 h AEL. (F) Average body weight measured from females (bars 1 to 3) or males (bars 4 to 6) of w¹¹¹⁸; DI-11-Gal4/+ and w¹¹¹⁸; DI-11-Gal4/UAS-AC76E flies at 330 AEL. The error bars represent standard deviations from at least three independent measurements. ***, P < 0.001 (Student's t test).